The recombinant DsP4H with l-proline as a substrate exhibited Km , kcat and kcat /Km values as much as 0.80 mM, 0.52 s-1 and 0.65 s-1 · To establish a green and environment friendly process for Hyp production, the proline 4-hydroxylase (DsP4H) from Dactylosporangium sp. The yr after Emil Fischer published the synthesis of proline from phthalimide propylmalonic ester. 4-Hydroxy-l-proline (trans-4Hyp) is broadly used as a priceless building block for the organic synthesis of many pharmaceuticals such as carbapenem antibiotics. Overexpression of a feedback inhibition-resistant γ-glutamic kinase mutant ProBG149K, deletion of a proline dehydrogenase to block l-proline degradation, overexpression of glutamate dehydrogenase to extend glutamate synthesis flux, the mutation of 6-phosphate gluconate dehydrogenase and glucose-6-phosphate-dehydrogenase within the pentose phosphate pathway to reinforce NADPH supply, the deletion of pyruvate aminotransferase to decrease the byproduct l-alanine synthesis, and weakening of α-ketoglutarate dehydrogenase to regulate the TCA cycle were combined to obtain ZQJY-9. Complexation: An Interesting Pathway for Combining Two APIs on the Solid State. Figure showing the metabolic pathway of L-proline through glutamate and ornithine. CRISPR-Cpf1-Assisted Engineering of Corynebacterium glutamicum SNK118 for Enhanced L-Ornithine Production by NADP-Dependent Glyceraldehyde-3-Phosphate Dehydrogenase and NADH-Dependent Glutamate Dehydrogenase. Metabolic engineering and flux analysis of Corynebacterium glutamicum for L-serine manufacturing.
Trans-4-hydroxy-L-proline manufacturing by the cyanobacterium Synechocystis sp. Metabolic engineering of Corynebacterium glutamicum for methionine production by eradicating feedback inhibition and growing NADPH level. Enhanced production of D-pantothenic acid in Corynebacterium glutamicum utilizing an environment friendly CRISPR-Cpf1 genome editing technique. Keywords: Corynebacterium glutamicum; SacB-assisted CRISPR-Cpf1 system; l-proline production; metabolic engineering. Composing Novel Diclofenac Potassium and l-Proline Salt Cocrystal as a method to extend Solubility and Dissolution. Crystal structure of novel dye-linked L-proline dehydrogenase from hyperthermophilic archaeon Aeropyrum pernix. Crystal of the new diclofenac-sodium-proline-monohydrate… Keywords: Cryopreservation; Ice crystal formation; Red blood cells; Trehalose; l-proline. Small molecule ice recrystallization inhibitors mitigate pink blood cell lysis throughout freezing, transient warming and thawing. ORTEP cell view and molecular construction graph of NDPM; Na… Recent advances of Cas12a functions in bacteria. Advances in Accurate Microbial Genome-Editing CRISPR Technologies. Zhang F, Wang JY, Li CL, Zhang WG. Zhang J, Qian F, Dong F, Wang Q, Yang J, Jiang Y, Yang S. Zhang J, et al. Li Y, Cong H, Liu B, Song J, Sun X, Zhang J, Yang Q. Li Y, et al. Zhang L, Xue X, Yan J, Yan LY, Jin XH, Zhu XH, He ZZ, Liu J, Li R, Qiao J. Zhang L, et al. Zhang F, et al.
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